自动化综合实验网络系统的构建与应用     

狄利明 《农机化研究》2006,(3):61-64

介绍了实验室现有的多个实验工艺流程装置以及控制系统的特点,描述了在实验室环境中应用常规控制系统、集散控制系统、现场总线控制系统与工业以太网控制系统实现对多个工艺流程装置的自动控制,以及研究自动化综合实验网络的构建、智能控制理论与方法的运用和教学实践的应用.  相似文献   

Effects of different lighting on reproductive system in depressive female rats     

DI Guan-teng  WANG Sha-li 《园艺学报》2016,32(3):510-515

AIM: To investigate the effects of different lighting on the reproductive system in depressive female rats. METHODS: Healthy adult female rats were randomly chosen as control group, and the depressive adult female rats in SPF grade were randomly divided into 5 groups(7 rats each):depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group. After 45 d of continuous illumination, the estrous cycle was observed by the vaginal exfoliated cells, and the organ indexes of ovary and uterus were calculated. The concentrations of estiadrol(E₂), prolactin(PRL), progesterone(PROG) and testosterone(T) in the serum were detected by ELISA, and the histopathological lesion of ovary was observed under microscope with HE staining. RESULTS: The estrous cycle exhibited serious disorder, the ovaries exhibited serious congestion, and the organ indexes of ovary and uterus and the concentrations of E₂, PRL, PROG and T decreased significantly in the rats in depressive model group compared with control group(P<0.05). The estrous cycle and histopathological damage of ovary were obviously improved, and the concentrations of E₂, PRL, PROG and T were significantly increased after the sulfur lamp lighting in the depressive female rats compared with depressive model group. No obvious change and improvement of the reproductive functions in the heat radiation lamp group, fluorescent lamp group and LED lamp group was observed. CONCLUSION: The reproductive functions of depressive female rats are improved by sulfur lamp lighting.  相似文献   

四川大豆疫霉毒力鉴定及资源抗性分析     

陈四维  张婷婷  肖丽  武晓玲  杨文钰 《中国油料作物学报》2016,(4):539-542

采用土壤诱捕和组织分离的方法,从四川省仁寿县和雅安市大豆根腐病发病田块采集的土壤中和病株上分离到4个大豆疫霉分离物RS1、RS2和YA1、YA2,用含有已知抗性基因的鉴别寄主进行毒力鉴定。结果表明YA1和YA2毒力公式相同,为1a,1b,1d,2,3c,属于同一致病型;RS1、RS2毒力公式分别为1a,1d,1k,3a,3c,4,5和1a,1b,1c,1d,1k,2,3c,5,分别为不同致病型。用3个致病型接种157份大豆资源,结果表明,抗病种质相对较少,占23.57%;地方品种抗病性较育成品种强,本研究可为大豆抗病性育种提供科学依据。  相似文献   

赭曲霉毒素A对肉鸡毒性作用的研究进展     

赭曲霉毒素A对肉鸡毒性作用的研究进展 《畜牧与饲料科学》2021,42(3):47-52

霉菌毒素污染是影响动物饲料安全的重要因素。赭曲霉毒素A（ochratoxin A,OTA）广泛存在于肉鸡饲料原料中。众多研究表明,OTA对肉鸡具有肾毒性、肝毒性、细胞毒性、遗传毒性、致癌性、致畸性、免疫毒性和肠道毒性等,给肉鸡养殖业带来了巨大的经济损失,也对动物和人类的健康造成威胁。对国内外OTA研究进展进行总结归纳,从诱导机体产生肠毒性、肝毒性、肾毒性和免疫毒性四方面阐述OTA对肉鸡的致毒机制,为深入研究OTA提供参考。  相似文献   

Aux/IAA基因家族鉴定及对大豆茎尖发育的调控作用分析     

刘婷婷  李艳燕  宁晓霜  刘志华  姜振峰  李文滨 《中国油料作物学报》2021,43(4):648

Aux/IAA 基因家族在植物茎尖发育过程发挥重要作用。为了探究大豆Aux/IAA 基因家族在大豆茎尖发育过程的调控作用，本文以拟南芥Aux/IAA 基因家族蛋白序列为参照鉴定了大豆全基因组Aux/IAA 家族基因，包括63个成员；然后以拟南芥、鹰嘴豆和大豆的Aux/IAA 家族为研究对象，比对这些基因全长氨基酸序列并构建进化树，结果表明三种作物的Aux/IAA 家族成员间亲缘关系差异明显，进化过程中同源重组频率不同；进而利用RNA-seq技术分析东农594（DN）、Charleston（CH）及二者杂交后代的RIL群体的高矮秆池（WH、WS）和F2群体的高矮秆池（JH、JS）的差异表达基因及其功能，共有17个Aux/IAA 基因在3组材料中差异表达。CHvsDN组有15个差异表达基因，JHvsJS组有2个，WHvsWS组只有1个；其中，Glyma.10G180100 在JHvsJS组和WHvsWS组均差异表达，这些结果为进一步研究大豆Aux/IAA 基因家族的功能及调控茎尖发育提供理论依据。  相似文献   

鸡源耐安普霉素大肠杆菌耐药表型研究     

高允乐  张秀英  底佳芳  陶娅 《中国兽药杂志》2005,39(9):9-11

进行了安普霉素耐药大肠杆菌耐药表型的研究.采用常规方法和生化鉴定管对有过安普霉素用药史的鸡场分离的鸡源病原性大肠杆菌进行鉴定,并用试管二倍稀释法测定其最低抑菌浓度(MIC),筛选出鸡源安普霉素耐药大肠杆菌;采用药敏纸片法研究了这些耐药菌对安普霉素等14种抗菌药物的敏感性.共筛选出7株对安普霉素耐药的鸡源性大肠杆菌,这些耐药菌株全部对安普霉素、妥布霉素、奈啶酸、多西环素和阿莫西林耐药;大部分对庆大霉素、链霉素、卡那霉素、壮观霉素也呈现耐药.对新霉素的耐药较低,对阿米卡星高度敏感.部分交叉耐药现象的存在揭示对安普霉素等氨基糖苷类药物产生耐药性的菌株,其他抗生素也可能对它们失去疗效.  相似文献   

Production of syringomycins and syringopeptins by Pseudomonas syringae pv. atrofaciens   总被引：1，自引：0，他引：1  

V. VASSILEV  P. LAVERMICOCCA  D. DI GIORGIO  & N. S. IACOBELLIS 《Plant pathology》1996,45(2):316-322

All virulent strains of Pseudomonas syringae pv. atrofaciens produce in vitro substances with syringomycin-like features. All strains inhibited the growth of Geotrichum candidum in the plate assay although the extent of their growth inhibition was variable.
Purification of bioactive culture extracts of a highly virulent strain by ion exchange chromatography (Whatman CM52) yielded a main fraction which inhibited the growth of Rhodotorula pilimanae and Bacillus megaterium , and was phytotoxic to tobacco and wheat plants. In particular, the injection of this fraction in the culm of wheat plants caused phytotoxic symptoms on leaves similar to those caused in nature by the pathogen. The further purification by HPLC of the above fraction gave rise to four main bioactive substances which have been identified by spectroscopic methods (FAB-MS) and amino acid analysis as syringomycin E, syringomycin G, syringopeptin 25A and syringopeptin 25B, toxic lipodepsipeptides thus far recognized to be produced by most strains of P. syringae pv. syringae . The injection of both syringomycin E and syringopeptin 25A in wheat leaves caused necrotic symptoms; however, syringopeptin 25A was at least six times more active than syringomycin E. The possible role of the toxins in the disease development on cereals and the need for a careful examination of pathogenetic and biochemical features of P. syringae pv. atrofaciens to establish the relationships of the two pathovars in the 'syringae group' are discussed.  相似文献   

荷斯坦奶牛耳组织成纤维细胞分离培养及冷冻保存方法研究   总被引：5，自引：0，他引：5  

王亮  刘婷婷  彭涛  朱海  汪汉卿  曹旭 《黑龙江畜牧兽医》2006,(5):9-12

试验对荷斯坦奶牛耳组织成纤维细胞的分离、体外培养及5种冷冻保存方法进行了研究。结果表明:用DMEM/F12 20%胎牛血清 100IU/mL双抗的完全培养液进行组织块培养,能获得良好的荷斯坦奶牛耳组织成纤维细胞原代培养物;成纤维细胞混合培养物经TrypsinEDTA(0.25%Trypsin,1mmol/LEDTA.4Na)消化所收集到的细胞主要为成纤维细胞,经2~3代传代,可得到纯化的荷斯坦奶牛耳组织成纤维细胞。纯化培养的成纤维细胞在冷冻保护剂和血清成分相同的条件下,选用5种不同的冷冻保存方法进行冷冻保存,其中使用70%细胞悬液 20%胎牛血清 10%DMSO的冷冻保存悬液,先在4℃预冷平衡0.5h,接着在液氮罐口的气态氮中悬挂4h,然后沉入液氮的方法冻存的细胞,解冻后,经台盼蓝染色后进行细胞活力分析,活细胞率为86.68%;培养48h后细胞贴壁率为86.40%,明显高于其他几种方法。  相似文献   

不同光增方式和周期对蛋鸡蛋品质和血液生化指标的影响   总被引：1，自引：0，他引：1  

黄仁录陈辉  潘栋邸科前  侯永刚 《中国畜牧杂志》2007,43(13):52-55

研究光增方式和光照周期对蛋鸡蛋品质和血液生化指标的影响。将20周龄海兰褐商品蛋鸡320只,随机分为8组,每组4个重复,每个重复10只鸡。试验光照周期采用渐增和骤增2种,光周期设11L∶13D、13L∶11D、15L∶9D、17L∶7D4个水平。结果表明:①不同光增方式对蛋形指数和哈氏单位有显著影响(P<0.05),以渐增条件下较好。不同光增方式对血液生化指标没有显著影响(P>0.05)。②不同光照周期对蛋黄颜色有显著影响(P<0.05),以15L∶9D处理组蛋黄颜色最好。不同光照周期对血液生化指标有一定影响,以13L∶11D处理组较好。  相似文献   

Implantation study on the potential of murine epidermal stem cell differentiation     

LIN Yi  LI Hai-biao  HUANG Jin-tao  ZENG Shan  DI Jing-fang  ZENG Yao-ying 《园艺学报》2006,22(12):2418-2423

AIM: To investigate the potential of murine epidermal stem cell (ESC) differentiation after seeded in a biodegradable carrier and implanted subcutaneously into syngeneic recipient mice. METHODS: ES cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a polyglycolic acid (PGA) net containing collagen gel, functioning as a cell carrier, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. RESULTS: The ESCs kept alive in the implant when observed under a fluorescent microscopy 3 weeks or longer after implantation, and could differentiate into hair follicle-like structure, glandular structure, and gave rise to additional structures displaying features resembling native dermis. No apparent rejection or severe side effects were observed at least 10 weeks post-implantation. CONCLUSION: It is feasible to use these ESCs as seed cells in the study to fabricate dermal equivalent having the potential to develop dermal appendages.  相似文献